KEANEKARAGAMAN TANAMAN Begonia cucullata Willd YANG DIINDUKSI DENGAN ETHYLMETHANE SULFONATE (EMS) BERDASARKAN VARIASI POLA PITA PROTEIN

Rizki,  Andy Soegianto1),  Estri Laras Arumingtyas2)

1)    Agricultural Faculty, Brawijaya University

2)    Biology, Faculty of Science, Brawijaya University

e-mail: khi_bio@yahoo.com

Web: http://:www.rizkibio.com

Abstract

Begonia cucullata is a herb plant, with erect stem, with no rhizome. Begonia used to become gardening plant, but its still has low phenotype variation. In order to increase plant variation and diversity may carried out with plant mutation. One of mutagen that can be used is Ethylmethane sulfonate. The aim of this research is to obtain new variation of B. cucullata and expected we can increase gardening plant variation in Indonesia. This research conducted using combination of EMS concentration variation (0.01%, 0.03%, and 0.05%) and length of soaking variation (4, 8, and 12 hours). Combination of concentration variation and length of soaking will replicated 5 times. This research has total 45 total samples and 3 controls. 

Result of the research showed differences in separation of protein band pattern on several samples, variation in thickness of protein band or existence of protein band in each samples. Soaking of plant with 0.05% EMS concentration and 12 hour length of soaking resulted in 17 bands, the same result also obtained with 0.01% EMS concentration for 8 hour.  Fewer bands (10) obtained on sample 28 and 26 that soaked with 0.01% and 0.05% EMS for 12 and 8 hour respectively. Fewer band (10 bands) observed in sample 26 and 28 with 0.05% EMS and 8 hour soaking time, 0.01% EMS and 12 hour soaking time, respectively. Highest total protein concentration obtained in sample 44 as big as 14.2 ppm. Lowest total protein concentration obtained in sample 26 as low as 14.2 ppm. Control sample showed 8 ppm total protein concentration. Binary data obtained from converting protein bands showed dendogram that indicated 25% mutation occurred.

Key Word : Begonia, Mutation, Protein, Ethylmethane Sulfonate

fulltext: khi_bio@yahoo.com